Genetics & Biology · The Living Address · Paper 12 of 23

Induction and Repression: The araBAD Operon

The Switch That Reverses

The switch that reverses · action at a distance · one protein, two signs · the ~200 bp loop

Stephen Daubney · The Daubney Foundation

araC turns the operon ON and OFFaction at a distance ≈ 200 bplooping is generalone regulator, two signs

The biology

The arabinose operon overturned the assumption that regulation is always repression: araC's product turns the operon on, yet the same protein can also act negatively — and its negative action, from a site about 200 base pairs away, revealed ‘action at a distance’ by DNA looping, now known to be general. Two uptake systems handle high and very low arabinose.

A reversible switch, and non-local control

Where the lac switch is a lock, the ara switch is one that can throw both ways — the same regulator opening or closing the coordinate as conditions change. And its control across 200 base pairs is the field acting non-locally on the address: a site far along the coordinate governs the promoter by folding the map, not by touching it. One protein with two signs is a single reader that can both open and shut the same address.

THE READING
Action at a distance is the field folding the address so a distant site governs the read — control is over the coordinate, not along the contiguous strand.

Where this departs from current science

Current science saysThe Force of Time says
A regulator is either an activator or a repressor.One reader can open or shut the same address — a reversible switch.
Action at a distance is loop-mediated protein contact.It is non-local control over a coordinate — the field folding the map.

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This paper, and any information drawn from it, may be used freely provided the reference attribution to Stephen Daubney and The Daubney Foundation is recognised.

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