Genetics & Biology · The Living Address · Paper 11 of 23

Gene Repression and the lac Operon

The First Switch

The first Τ-switch · the repressor lock · DNA looping · the CRP·cAMP gate

Stephen Daubney · The Daubney Foundation

lac = the first genetic switchrepressor loops the DNACRP·cAMP = positive gateon only if lactose & no glucose

The biology

The lac operon is the founding story of gene regulation. A repressor protein binds the operator and blocks the polymerase; when lactose appears, its product binds the repressor and lets go, and the genes for using lactose are transcribed. Positive control adds a gate: CRP·cAMP activates the promoter, and cAMP is high only when glucose is scarce — so lac is fully on only when lactose is present and glucose absent. Repressor bound at two operators at once loops the DNA, tightening the lock.

The first switch on the address

Here is the first Τ-switch — a gate that decides whether a stretch of the address is read. A regulator acts on one operon, not on all genes alike, so it is a way of choosing which coordinate is expressed and when. The DNA loop is the field folding the address so that one site controls another across a distance; the two-signal logic (lactose and glucose) is a coordinate read only under the right conditions of the register.

THE READING
Regulation is a gate on the address: the repressor holds the coordinate shut, the inducer opens it, and the loop lets one site govern another — the field reading its own map conditionally.

Where this departs from current science

Current science saysThe Force of Time says
The operon is a biochemical feedback circuit.It is the first Τ-switch — a conditional gate on which part of the address is read.
DNA looping is protein-mediated cooperativity.It is the field folding the address so one site governs another across a distance.

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This paper, and any information drawn from it, may be used freely provided the reference attribution to Stephen Daubney and The Daubney Foundation is recognised.

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