Genetics & Biology · The Living Address · Paper 18 of 23

Site-Specific Recombination: Lambda Integration and Excision

A Place in the Book

Reversible integration · Int and Xis · the address written in and out · Lk conserved

Stephen Daubney · The Daubney Foundation

site-specific recombinationInt inserts · Int+Xis excisedirection is controlledreversibility = dΣΤ = 0

The biology

A lysogen integrates its DNA at a specific chromosomal site so it co-replicates with the host, lost only about once per million divisions. Integration is site-specific recombination: Int recombines the phage and host sites to insert; Int together with Xis runs the exact reverse to excise. The direction is controlled — infection integrates, induction excises.

A place written into the book, and out again

This is the address written into the book at a chosen place and taken out again cleanly — a reversible edit of the coordinate. That the same machinery runs forward and backward, and that excision restores the original exactly, is conservation: the linking number and the sequence are preserved across the round trip, an instance of dΣΤ = 0. The field can add a coordinate to another's address and later remove it without loss.

THE READING
Reversible integration is a conserved edit of the address: written in by Int, taken out by Int and Xis, the coordinate preserved across the round trip — dΣΤ = 0 in a recombination.

Where this departs from current science

Current science saysThe Force of Time says
Integration/excision are opposed enzymatic recombinations.They are a reversible, conserved edit of the address — the round trip preserves the coordinate.
Directionality is set by Xis availability.It is the controlled writing-in and taking-out of a coordinate without loss.

Open the full paper (PDF) ↗

This paper, and any information drawn from it, may be used freely provided the reference attribution to Stephen Daubney and The Daubney Foundation is recognised.

The same single substance — time — writes the address of a cell, the wavelength of a spectral line, and the orbit of a planet. If this stirred your curiosity, the whole weave is waiting.

Read the whole theory of the Universal Force of Time →